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Microscopy and Histology Suite

Our Mass Spectrometry facility houses an Agilent 6540 Quadrupole Time-Of-Flight (QTOF) mass spectrometer with standard and microfluidic chromatography options.

In addition, an Agilent 5495C Triple Quadrupole (QQQ) mass spectrometer with standard chromatography options and on-line SPE capability has just been installed and commissioned.

Equipment

The Agilent 6540 QTOF mass spectrometer is a mass accurate instrument capable of less than 3ppm error and a mass range of up to 10000 Daltons. Mass resolution is 45000 FWHM at 1000 Daltons. The 6540 QTOF utilises analog to digital (ADC) conversion technology in its detector setup, providing uncompromising mass resolution at fast scan speeds with a large dynamic range.

The 6540 QTOF utilises a 1290 Infinity liquid chromatography (LC) system with maximum pressures up to 1200 bar and 5ml/min. This system is designed to handle column stationary phases with sub 2µm particle technology for fast, high-resolution chromatography. This LC system couples to a Dual electrospray ionisation (ESI) Jetstream Source for mid-analysis mass accuracy correction.

The Agilent 8495C triple quadrupole mass spectrometer (QQQ) is Agilent’s most sensitive triple quadrupole system and has femtogram on column sensitivities.

The 6495C QQQ utilises a 1290 Infinity liquid chromatography (UHPLC) system with maximum pressures up to 1200 bar and 5ml/min. This system is designed to handle column stationary phases with sub 2µm particle technology for fast, high resolution chromatography. This system has very fast MS/MS operation, which can better identify compounds in complex systems and establish faster analysis methods.

The 6495C QQQ also has a quaternary 1260 UHPLC pump and on line SPE valve configuration to automate sample preparation and sample cleanup.

HPLC

Switching between users for different analytical applications is relatively simple, through either software settings or switching between separation columns.

Agilent 6540 QTOF mass spectrometer

Training

Training is a prerequisite to accessing our Mass Spectrometry shared equipment. Training is provided by our facility staff and is obligatory regardless of experience using similar instruments. To ensure consistency in training, users are not permitted to train other users. Training on the 6540 QTOF takes approximately 16 hours (usually over three days) and entails theory, practical and software/processing training. Training on the HPLC is also available through our Mass Spectrometry facility staff.
 

Induction 6540 QTOF 

Theory
Theory covers basic principles of sample preparation, liquid chromatography, and mass spectrometry principles with grounding in first principles of each technology. Theory will cover basics of most techniques users are exposed to within our Mass Spectrometry facility.
 

Practical
Users are trained in developing a method for caffeine quantification in food/drink items in an interactive setting. Users are shown basic start-up and maintenance procedures and walked through method development for chromatography and mass spectrometry. 

Software/processing
Users are familiarised with basic software systems utilised by the 6540 QTOF system and process data collected in the practical component to detect and quantify caffeine. In addition, more complex method development and screening protocols and methods specific to each user’s project are discussed.
 

Follow-up training
After induction and discussion over each user’s specific application, method development for each user’s project is done under supervision. During this time, users are trained in project-specific applications of shared equipment. After method development and users are judged competent, further analysis is performed independently.
 

Training is a prerequisite to accessing our Mass Spectrometry shared equipment. Training is provided by our facility staff and is obligatory regardless of experience using similar instruments. To ensure consistency in training, users are not permitted to train other users. Training on the 6540 QTOF takes approximately 16 hours (usually over three days) and entails theory, practical and software/processing training. Training on the HPLC is also available through our Mass Spectrometry facility staff.
 

Induction 6540 QTOF 

Theory
Theory covers basic principles of sample preparation, liquid chromatography, and mass spectrometry principles with grounding in first principles of each technology. Theory will cover basics of most techniques users are exposed to within our Mass Spectrometry facility.
 

Practical
Users are trained in developing a method for caffeine quantification in food/drink items in an interactive setting. Users are shown basic start-up and maintenance procedures and walked through method development for chromatography and mass spectrometry. 

Software/processing
Users are familiarised with basic software systems utilised by the 6540 QTOF system and process data collected in the practical component to detect and quantify caffeine. In addition, more complex method development and screening protocols and methods specific to each user’s project are discussed.
 

Follow-up training
After induction and discussion over each user’s specific application, method development for each user’s project is done under supervision. During this time, users are trained in project-specific applications of shared equipment. After method development and users are judged competent, further analysis is performed independently.
 

Bookings 

Access to shared equipment is strictly through our Mass Spectrometry facility staff. Please contact staff if you are considering usage of shared equipment.

Attribution and authorship 

All data generated using the shared equipment should acknowledge our Mass Spectrometry facility. In addition, where any member of our Mass Spectrometry facility has made substantial intellectual contributions to the design of your experiment, they should qualify among the authorship list. 

Training and access

All users must complete facility-specific training before gaining independent access to shared research equipment. Training includes:
1. General Inductions: Covering fundamental principles, equipment operation, and safety protocols.
2. Instrument-Specific Training: Hands-on instruction tailored to researchers' specific applications.
3. Refresher & Advanced Training: Ongoing skill development and troubleshooting support.

Bookings and facility use

Licensed users have access to online booking systems for equipment scheduling.
Contact facility staff for training, troubleshooting, or equipment-specific queries.

Attribution and authorship

All research using Curtin MRI facilities must acknowledge the relevant facility in publications. Where significant intellectual contributions are made by facility staff, authorship should be considered.

Facilities, contact and support

For inquiries or feedback contact:

Dr Rob Steuart
Facilities Manager
R.Steuart@curtin.edu.au
Tel: 9266 2342